近日，本单位合作机构奥根诊断完成了科研成果数据更新。奥根诊断是国内首家拥有供体来源的游离DNA（ddcfDNA）在器官移植方面应用技术的公司，国内合作器官移植中心100多家，目前已累计完成15000+份ddcfDNA样本的检测，是最大的“中国器官移植ddcfDNA基因数据库”。经过7年在ddcfDNA检测方向的深耕，公司已构建起覆盖技术研发、临床验证、数据积累及国际标准输出的完整生态体系，目前已获授权核心发明专利19项、软件著作权23项，合作发表ddcfDNA相关论文20篇（SCI 15篇），并有多项合作研究成果受邀在美国器官移植年会(ATC)、欧洲器官移植年会(ESOT)和国际器官移植年会(TTS)上展示。
以下为奥根诊断合作研究成果更新详情：
合作发表文章(更新至2025年3月)
1) Continuous Monitoring of Donor-Derived Cell-Free DNA:Guiding Diagnosis and Management of Subclinical Rejection
Dongrui Cheng，Cuello Garcia Haider，Xue Li，Kenan Xie，Xuefeng Ni，Yang Zhou，Sicheng
Wu，Xiaohui Xu，Tingya Jiang，Jinsong Chen.
https://www.ectrx.org/detail/current/2025/23/2/0/158/0
2) Diagnostic value of three urine miRNAs composite score in BK virus nephropathy
Zhang Z, Cao C, Garcia Haider C, Wang Y, Zhu Y, Abdelhak O, Liu H, Yu C, Chang S, Zhang W, Jiang T, Zhou Y.
https://doi.org/10.1016/j.cca.2025.120162
3) Donor-derived cell-free DNA-based liquid biopsies to determine future kidney transplant rejection
Wang W, Haider CG, Wang Y, Zhang Z, Liu Y, Xue F, Liu H, Jiang T, Cao J, Zhou Y.
https://doi.org/10.23876/j.krcp.23.286
4) Analysis of the primary factors influencing donor derived cell-free DNA testing in kidney transplantation
Cao C, Yuan L, Wang Y, Liu H,Cuello Garcia H, Huang H, Tan W,Zhou Y, Shi H and Jiang T.
https://doi.org/10.3389/fimmu.2024.1435578
5) Cell-Free Mitochondrial DNA: An Upcoming Non-Invasive Tool for Diagnosis of BK Polyomavirus-Associated Nephropathy
Guo L, Luo S, Wang X, Zhang N, Cheng Y, Shen J, Chen J, Wang R.
https://doi.org/10.3390/biom14030348
6) Differentiation between lung allograft rejection and infection using donor-derived cell-free DNAand pathogen detection by metagenomic next-generation sequencing
Chunrong Ju, Lulin Wang, Peihang Xu, Xiaohua Wang, Dong Xiang, Yu Xu, Xin Xu, Rongchang
Chen,Jianxing He.
DOI: 10.1016/j.heliyon.2023.e22274
7) Application of plasma donor-derived cell free DNA for lung allograft rejection diagnosis in lung transplant recipients
Chunrong Ju, Lulin Wang, Peihang Xu, Xiaohua Wang, Dong Xiang, Yu Xu, Xin Xu, Rongchang
Chen,Jianxing He.
https://bmcpulmmed.biomedcentral.com/articles/10.1186/s12890-022-02229-y
8) Using Both Plasma and Urine Donor-Derived Cell-Free DNA to Identify Various Renal Allograft Injuries
Xu-Tao Chen, Jiang Qiu, Zi-Xuan Wu, Hui Zhang, Tong Chen, Shi-Cong Yang, Guo-Dong Zhao, Yu He, Xue Shen, Jin-Quan Luo, Yang Huang, Chang-Xi Wang, Li-Zhong Chen, Cheng-Lin Wu, Gang
Huang.
Clinical Chemistry, hvac053, https://doi.org/10.1093/clinchem/hvac053
9) Analysis of cell-free fetal DNA in 16,843 pregnant women from a single center in China using targeted sequencing approach
Yang Zhou , Yinfeng Wang , Frank Peprah Addai , Xiaofeng Li , Xiuwen Zhang , Haitao Liu , Gongda Yang , Fengbo Zeng , Tingya Jiang , Junling Liu.
https://doi.org/10.1016/j.placenta.2022.03.126
10) Plasma donor-derived cell-free DNA levels are associated with the inflammatory burden and macrophage extracellular trap activity in renal allografts.
Luying Guo , Jia Shen , Wenhua Lei , Pengpeng Yan , Meifang Wang , Qin Zhou , Huiping Wang , Jianyong Wu , Jianghua Chen.
https://www.frontiersin.org/articles/10.3389/fimmu.2022.796326/abstract
11) Dynamics of Donor-Derived Cell-Free DNA at the Early Phase After Pediatric Kidney Transplantation: A Prospective Cohort Study
Nie W, Su X, Liu L, Li J, Fu Q, Li X, Wu C, Wang J, Deng R, Chen E, Yang S, Li S, Zhang H, Wang C.
Front Med (Lausanne). 2022 Jan 7;8:814517.
12) Donor-derived cell-free DNA: An independent biomarker in kidney transplant patients with antibody-mediated rejection
Cheng D, Liu F, Xie K, Zeng C, Li X, Ni X, Ge J, Shu L, Zhou Y, Shi H, Liu H, Chen J.
Transpl Immunol. 2021 Dec;69:101404.
13) Urinary donor-derived cell-free DNA as a non-invasive biomarker for BK polyomavirus nephropathy
Shen J, Guo L, Lei W, Liu S, Yan P, Liu H, Zhou J, Zhou Q, Liu F, Jiang T, Wang H, Wu J, Chen J, Wang R.J
Zhejiang Univ Sci B. 2021 Nov 15;22(11):917-928. English.
14) A novel urine cell-free DNA preservation solution and its application in kidney transplantation
Zhou Q, Liu F, Guo L, Chen R, Yuan X, Li C, Shu L, Liu H, Zhou Y, Wu Y, Shi H, Zhao H, Jiang T.
Nephrology (Carlton). 2021 Aug;26(8):684-691.
15) The role of donor-derived cell-free DNA in the detection of renal allograft injury
Zhou Y, Cheng D, Jiang T.
Nephrol Ther. 2021 Feb;17(1):12-17.
16) Preliminary clinical experience applying donor-derived cell-free DNA to discern rejection in pediatric liver transplant recipients
Zhao D, Zhou T, Luo Y, Wu C, Xu D, Zhong C, Cong W, Liu Q, Zhang J, Xia Q.Sci Rep. 2021 Jan 13;11(1):1138.
17) Prognostic value of the donor‐derived cell‐free DNA assay in acute renal rejection therapy: A prospective cohort study
Shen J, Guo L, Yan P, Zhou J, Zhou Q, Lei W, Liu H, Liu G, Lv J, Liu F, Huang H, Dong W, Shu L, Wang H, Wu J, Chen J, Wang R.Clin Transplant. 2020 Oct;34(10):e14053.
18) Urine Donor–Derived Cell-Free DNA Helps Discriminate BKPyAN in Kidney Transplant Recipients With BK Polyomavirus Infection
Chen XT, Chen WF, Li J, Deng RH, Huang Y, Yang SC, Chen PS, Jiang TY, Liu HT, Wang CX, Chen LZ, Qiu J, Huang G.
Front Immunol. 2020 Aug 19;11:1763.
19) 嵌合抗原受体调节性T细胞免疫疗法在器官移植中的应用
吴俣 李佩璐 葛军 蒋廷亚, 《器官移植》2020,11(5):547-552
20) Donor-Derived Cell-Free DNA in Urine Identifies BKVN with Graft Injury in BK Viruria Kidney Transplant Recipients
Huang G, Chen X, Yang S, Chen W, Chen P, Hou X, Jiang T, Liu H, Li X, Wang C.
[abstract].Am J Transplant. 2019; 19 (suppl 3).
21) Donor-derived Cell-free Dna In Donor Specific Antibody Positive Kidney Transplant Recipients
Cheng D, Li X, Jiang T, Liu H, Zhou Y, Shi H.
[abstract].Am J Transplant. 2019; 19 (suppl 3).
22) Periphery Blood ddcfDNA Can Significantly Differentiate Acute Humoral Rejection from Banff’s Type I Acute Rejection in Kidney Transplantation
Wang R, Jiang T, Shen J, Ge J, Lei W, Yang X, Deng H, Kong W, Zhou J.
[abstract].Am J Transplant. 2019; 19 (suppl 3).
23) 供体来源游离DNA在移植肾损伤中的研究进展
蒋廷亚 周阳 程东瑞，《中华器官移植杂志》2019年9月第40卷第9期
24) Dynamics of early post-operative plasma ddcfDNA levels in kidney transplantation: a single-center pilot study
Shen J, Zhou Y, Chen Y, Li X, Lei W, Ge J, Peng W, Wu J, Liu G, Yang G, Shi H, Chen J, Jiang T, Wang R.
Transpl Int. 2019 Feb;32(2):184-192.
25) A Noninvasive and Donor-independent Method Simultaneously Monitors Rejection and Infection in Patients With Organ Transplant
Zhou Y, Yang G, Liu H, Chen Y, Li X, Ge J, Wang X, Niu H, Dong W, Jiang T, Shi H, Peng L, Liu L.
Transplant Proc. 2019 Jul-Aug;51(6):1699-1705.
26) 器官移植术后病原体感染检测研究进展
王啸晨 葛军 陈雅文 蒋廷亚 黎小锋 杨功达 周阳 王仁定.
《器官移植》2018,9(06):474-477.
27) 游离DNA在器官移植急性排斥反应检测中的应用
陈雅文 蒋廷亚 杨功达 周阳 唐笑天 刘磊，
《中华器官移植杂志》 2018,39(1):58-61.
合作国际会议口头报告(ATC/ESOT/TTS)
1) Simultaneously detection of ddcfDNA in plasma and urine assist to determine the type of rejection in renal transplant patients[abstract]. TTS 2020: Oral Presentation 310.2. (https://virtual.tts.org/virtual/lecture/285).
2) Urine Donor-derived Cell-free is Valuable for Predicting BK Polyomavirus-associated Nephropathy in Kidney Transplant Recipients[abstract]. Am J Transplant. 2021; 21 (suppl 3). (https://atcmeetingabstracts.com/abstract/urine-donor-derived-cell-free-is-valuable-for-predicting-bk-polyomavirus-associated-nephropathy-in-kidney-transplant-recipients/).
3) Donor derived cell-free DNA in donor specific antibody positive kidney transplant recipient[abstract]. ESOT 2019: Oral Presentation; （https://onlinelibrary.wiley.com/doi/epdf/10.1111/tri.13507 ）
4) Detecting donor derived cell free DNA levels to identify BKVAN and BKV infection combined TCMR[abstract]. ESOT 2019: Oral Presentation; 4395346
5) Simultaneous quantification of ddcfDNA and noninvasive genotyping of donor’s HLA in renal transplant patients by next-generation sequencing[abstract]. ESOT 2019: Oral Presentation; 4395393.
6) Detecting donor derived cell-free DNA in urine identify BKVN with graft injury in BK viruria kidney transplant recipients.ESOT 2019: Oral Presentation; 4396189